Effect of soil organic carbon loss on the stability and structure of microaggregates: First insights from an organic carbon depletion field trial in a loess soil

Soil microaggregates are considered to play an important role in soil functioning and soil organic carbon (SOC) is of great importance for the formation and stabilization of these aggregates. The loss of SOC can occur, for example, after a change in land use and may lead to a decreased aggregate stability, which makes soils vulnerable to various threats, such as erosion or compaction. It is therefore important to understand the effect of SOC loss on aggregate stability in order to better understand and preserve the functioning of healthy soils.

We sampled two adjacent plots from a loess soil in Selhausen (North Rhine-Westphalia, Germany) in November of 2019 and measured aggregate stability and architecture of soil microaggregates. One plot was kept free from vegetation by the application of herbicides and by tillage (to a depth of 5 cm) from 2005 on, while the other plot was used for agriculture (conventional tillage). Over the course of 11 years, the SOC concentration in the bulk soil was reduced from 12.2 to 10.1 g SOC kg^-1 soil. We took 10 undisturbed soil cores from two depths of each plot (Ap and Bt horizons).

The stability of aggregates against hydraulic and mechanical stresses was tested using the widespread wet sieving approach and a newly developed dry crushing approach. Isolated microaggregates gained from the latter procedure were tested against tensile stress by adapting a crushing test in a load frame to the microaggregate scale. To shed light on the effect of a decreased SOC content on microaggregate structure, we scanned several microaggregates with a high-resolution computed tomography scanner (Zeiss Xradia 520 versa) at sub-micron resolutions and analyzed the features of their pore systems.

This will give us valuable insights into the interplay of mechanical and physicochemical stability, as well as the structural properties of microaggregates with regard to SOC depletion. The consequences for various soil functions provided by microaggregates, like the habitat function for microorganisms or their capacity to store and transport gas, water and nutrients, are discussed.