EGU21-10994
https://doi.org/10.5194/egusphere-egu21-10994
EGU General Assembly 2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Silicon‐mediated manganese tolerance of cucumber: the apoplastic modulation

Jelena Dragisic Maksimovic1, Milos Mojovic2, and Vuk Maksimovic1
Jelena Dragisic Maksimovic et al.
  • 1Institute for Multidisciplinary Research, University of Belgrade, Life Science, Belgrade, Serbia (draxy@imsi.rs)
  • 2Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12, 11000 Belgrade, Serbia (milos@ffh.bg.ac.rs)

An impressive body of Si research could be found in the literature despite the fact that, from a biochemical perspective, Si is a “monotonous” element largely uncharged and unreactive at physiological pH (forming mostly silicates and SiO2 polymers). However, the detailed role of Si in plants remains unexploited, particularly the potential for its practical application. One of the main properties of Si intensively explored is the protection mechanism(s) against biotic and abiotic stresses, especially heavy metal stress. To investigate the effect of Si application on the Mn binding potential of the leaf apoplast, cucumber plants were grown in nutrient solutions with optimal (0.5 µM) or excessive (100 µM) Mn concentrations with or without Si supply to roots. Leaves were subjected to fractionated extraction of Mn revealing a relative distribution of Mn fractions in cucumber leaves: water-extractable (WE) Mn represents the soluble fraction in the cell walls; the protein-bound (PB) Mn fraction originates mostly from the symplast; while the cell wall-bound (CWB) Mn fraction represents Mn which is fixed to the wall structure. After the high Mn supply (100 µM), the concentration of WE Mn was 10-fold higher compared to control, while the relative proportion of the WE Mn fraction decreased from 56% in control to 23% in high Mn treatment. Si application did not affect WE and PB Mn fractions in the control treatment but significantly decreased these fractions in the high Mn treatment. On the other hand, the CWB Mn significantly increased in the leaves of Si-fed plants. Data obtained by fractionated Mn extraction are consistent with the relative proportion of free and bound Mn, estimated from the recorded electron paramagnetic resonance (EPR) signals of Mn2+. The EPR spectrum of a high spin Mn2+ showed the characteristic six hyperfine lines whose intensity correlated with Mn treatments and, consequently, leaf concentrations of Mn. The results presented here demonstrated that Si supply increased the Mn binding properties of leaf cell walls in cucumber plants with simultaneously decreasing of the free apoplastic Mn2+, indicating the protective role of Si in smothering harmful (inter)actions of free Mn2+ within plant tissue. Taken together, the leaf apoplast plays the central role in modulation of Mn toxicity and Si enhanced Mn tolerance in cucumber.

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Contract No. 451-03-68/2020-14/200053).

How to cite: Dragisic Maksimovic, J., Mojovic, M., and Maksimovic, V.: Silicon‐mediated manganese tolerance of cucumber: the apoplastic modulation, EGU General Assembly 2021, online, 19–30 Apr 2021, EGU21-10994, https://doi.org/10.5194/egusphere-egu21-10994, 2021.

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