The European countries are often recognised as the cradle of some of the world’s most important cultural heritage in stone. The cultural, artistic and social importance of stone monuments and lithic works of art evidences the general need to safeguard our praiseworthy cultural heritage. Unfortunately, we are confronted with some problems concerning their conservation, such as the increase of atmospheric contamination, the complex interactions between physical, chemical and biological factors, vandalism, lack of maintenance, and inefficient conservation treatments. This session will focus on the novel approaches that have been recently developed in the field of stone cultural heritage. The new emerging technologies, together with the variety of strategies, methodologies and biotechnological approaches available today show the wide range of possibilities that can be applied to stone heritage conservation. We invite studies devoted to: (i) novel tools for the identification of microorganisms and metabolites responsible for stone biodeterioration; (ii) biomaterials used for the preservation of granite and limestone materials; (iii) natural products from plants or microorganisms as innovative bioactive compounds for controlling biodeterioration; (iv) biotechnological approaches for the preservation of stone-built heritage and removal of sulphates, nitrates or organic substances from stone walls; (v) bioremediation strategies for building restoration. Experimental design setups, laboratory-based assays and field tests are also welcomed.