EGU2020-1929
https://doi.org/10.5194/egusphere-egu2020-1929
EGU General Assembly 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Cu-NPs combating fungicide resistance: effectiveness and synergy against B. cinerea

Anastasios Malandrakis1,2, Nektarios Kavroulakis3, and Constantinos Chrysikopoulos1
Anastasios Malandrakis et al.
  • 1Technical University of Crete, School of Environmental Engineering, Chania, Greece
  • 2Pesticide Science Laboratory, Department of Crop Science, Agricultural University of Athens,75 Iera Odos, 118 55 Athens, Greece.(tasmal@aua.gr)
  • 3Hellenic Agricultural Organization “Demeter”, Institute for Olive Tree, Subtropical Plants and Viticulture, Agrokipio-Souda, 73164 Chania, Greece.

The potential of copper nanoparticles (Cu-NPs) alone or in combination with conventional fungicides against sensitive and resistant to fungicides Botrytis cinerea isolates was assessed in vitro and in vivo. DNA sequencing revealed the E198A resistance mutation in the β-tubulin gene in three B. cinerea isolates highly resistant to benzimidazoles (BEN-R), thiophanare methyl (TM), and the G143A mutation in the cytb gene in four isolates highly resistant to the QoI pyraclostrobin (PYR-R). Cu-NPs could effectively control sensitive and resistant isolates. A synergistic effect between Cu-NPs and TM both in vitro and in vivo was observed in the case of benzimidazole sensitive   isolates while an additive effect was observed in BEN-R isolates. The above observed synergistic action could be attributed to increased TM availability in the target site as indicated by the positive correlation observed between TM and TM+Cu-NPs treatments. A positive cross sensitivity and antagonistic action between Cu-NPs and NaCl suggested that copper ions contribute in the fungitoxic action of Cu-NPs, at least partly, since no correlation between Cu(OH)2 and Cu-NPs sensitivity was found. The co-application of Cu-NPs with the oxidative phosphorylation inhibitor fluazinam (FM) resulted in a synergistic action  in all isolates regardless resistance phenotype, indicating a ATP-dependent mechanism of toxic action of Cu-NPs. Cu-NPs combined with conventional fungicides can aid in the design and implementation of eco friendly, sustainable management strategies by reducing fungicide use and combating resistance against B. cinerea.

How to cite: Malandrakis, A., Kavroulakis, N., and Chrysikopoulos, C.: Cu-NPs combating fungicide resistance: effectiveness and synergy against B. cinerea, EGU General Assembly 2020, Online, 4–8 May 2020, EGU2020-1929, https://doi.org/10.5194/egusphere-egu2020-1929, 2020

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