EGU General Assembly 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

The effect of stream microbial inoculation on in-stream carbon processing

Jakob Schelker1,2, Florian Caillon1,2, Katharina Besemer2, Peter Peduzzi1, and Astrid Harjung1,2
Jakob Schelker et al.
  • 1University of Vienna, Dept. of Limnology & Bio-Oceanography, Vienna, Austria (
  • 2WasserCluster Lunz GmbH, Lunz am See, Austria

Hydrological events mobilize chemically diverse dissolved organic matter (DOM) from soils to streams. Further, such events can also cause an influx of soil microbial life into fluvial systems. Here we present results from the HYDRO-DIVERSITY project, which aims to investigate the dynamic transfer of DOM and microbial life from catchment soils to streams, as well as their downstream fate. We studied the microbial community composition and DOM quality using 16S Illumina sequencing and fluorescence and absorbance spectroscopy. Data from small streams showed strong changes in DOM composition and in the microbial community delivered from soils during hydrological events. Moreover, we performed a flume experiment, in which soil microbial inoculation and the processing of DOM across different biofilm ages were evaluated. As such, biofilm age did not directly affect the establishment of soil microbes in the stream ecosystem. However, in-stream processing of soil DOM appeared to be affected by the inoculation event. This poses the fundamental question, if the processing of DOM in streams and rivers depends on the transient presence of specific soil microbes in stream ecosystems. Overall our results show that soils provide a dynamic and relevant influx of microbes and DOM to first order streams and that this dynamic influx likely affects microbial community dynamics of downstream fluvial networks as well as in-stream DOM processing. 

How to cite: Schelker, J., Caillon, F., Besemer, K., Peduzzi, P., and Harjung, A.: The effect of stream microbial inoculation on in-stream carbon processing, EGU General Assembly 2020, Online, 4–8 May 2020, EGU2020-20555,, 2020