EGU2020-22121
https://doi.org/10.5194/egusphere-egu2020-22121
EGU General Assembly 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Comparative Prevalence and Provenance of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes in Tropical Rivers of Sri Lanka and India

Manish Kumar1, Tushara Chaminda2, Sulfikar Hanafi3, Arbind Patel1, Payal Mazumder4, Alok Thakur1, Ryo Honda5, and Himaya Sewwandi
Manish Kumar et al.
  • 1Department of Earth Sciences, Indian Institute of Technology, Gandhinagar, India (manish.kumar@iitgn.ac.in)
  • 2Department of Civil and Environmental Engineering, University of Ruhuna, Galle, Sri Lanka
  • 3Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
  • 4Centre for the Environment, Indian Institute of Technology Guwahati, Assam-781039, India
  • 5Faculty of Environmental Design, Institute of Science and Engineering, Kanazawa University, Japan

Abstract:

Prevalence and provenance of antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARG) and metal concentrations were compared in river Kelani of Sri Lanka and Sabarmati and Brahmaputra of India. The prevalence of E. coli was 10-27, 267-76,600 and <50 CFU ml-1 in aforementioned rivers, respectively. Isolated E. coli colonies were subjected to resistance test with norfloxacin (NFX), ciprofloxacin (CIP), levofloxacin (LVX), kanamycin monosulphate (KM), tetracycline (TC), and sulfamethoxazole (ST). The isolates were predominantly multi-antibiotic resistant, with greater resistance to TC and ST. Brahmaputra River showed greater resistance to all tested antibiotics. Sabarmati River showed higher resistance to TC and ST than Kelani. Genes conferring resistance to tetracyclines, sulphonamides, b-lactams and fluoroquinolones were common. ARG, gyrA, tetW, sul1 and ampC were detected in Kelani River, additionally, aac-(6’)-1b-cr, and blaTEM were detected in Brahmaputra River. In both countries, less polluted segments exhibited more copies of ARG. Faecal contamination was decoupled from percentage antibiotic resistance and metal contamination, suggesting to separate of hospital waste from domestic waste with specific guidelines.

 

Keywords: Antibiotic Resistance; Brahmaputra; E. coli; Kelani River; Sabarmati, Gene

How to cite: Kumar, M., Chaminda, T., Hanafi, S., Patel, A., Mazumder, P., Thakur, A., Honda, R., and Sewwandi, H.: Comparative Prevalence and Provenance of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes in Tropical Rivers of Sri Lanka and India, EGU General Assembly 2020, Online, 4–8 May 2020, EGU2020-22121, https://doi.org/10.5194/egusphere-egu2020-22121, 2020