EGU2020-7121
https://doi.org/10.5194/egusphere-egu2020-7121
EGU General Assembly 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Stabilization of labile carbon in soil microbial biomass and necromass – A question of nitrogen deficiency?

Nele Meyer1,2, Outi-Maaria Sietiö2, Sylwia Adamczyk2, Christina Biasi3, Per Ambus4, Kevin Mganga2, Rashmi Shrestha2, Subin Kalu2, Angela Martin2, Bruno Glaser5, and Kristiina Karhu2
Nele Meyer et al.
  • 1Department of Soil Ecology, University of Bayreuth, Bayreuth, Germany (nele.meyer@uni-bayreuth.de)
  • 2Department of Forest Sciences, University of Helsinki, Helsinki, Finland
  • 3Biogeochemistry Research Group, University of Eastern Finland, Kuopio, Finland
  • 4Department of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark
  • 5Institute of Agronomy and Nutritional Sciences, Soil Biogeochemistry, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany

It has been assumed for a long time that stable soil organic carbon (SOC) results from selective preservation of plant residues. Yet, a new paradigm points to a more active role of microorganisms in building SOC storage. In this context, even labile C, such as sugars, may persist in soil for a long time due to their incorporation into microbial biomass and ultimately necromass. The latter is considered as a relatively stable pool. However, little is known about the cycling of labile C through the microbial biomass and the turnover time of its residues. Unraveling the mechanisms and regulating factors would be critical for understanding SOC stabilization in soil.

We assume that the fate of labile C is mainly driven by microbial nitrogen (N) demand and supply. Specifically, we hypothesize that (1) high N demand forces microbes to decompose N-rich substances (“microbial N mining”), such as amino sugars, leading to a rapid turnover of microbial necromass, and that (2) labile C is stabilized in microbial necromass when N demand is met.

To investigate these hypotheses, we set up a greenhouse pot experiment including four treatments: (1) bare soil, (2) bare soil+N, (3) tree, and (4) tree+N. The soil is a sandy and nutrient poor forest soil from southern Finland. Trees are 1 m high pines (Pinus Sylvestris), which are supposed to induce microbial N deficiency by exuding easily degradable C compounds and by competing with microbes for mineral N. In order to follow to fate of labile C, we added trace amounts of 13C labeled glucose to the soil (4 replicates per treatment). As a control to account for background variations in 13C, we added 12C glucose to another set of pots (4 replicates per treatment). Up to now, we sampled the soil 1 day, 3 days, 8 days, 1 month, 3 months, 6 months, 9 months, and 1 year after glucose addition. Measurements of the 13C recovery in soil, microbial biomass, water extractable C, PLFA, amino sugars, and DNA are in progress.

First results indicate that the largest loss of 13C tracer occurred in the unfertilized tree treatment, i.e., where N demand was high but N supply was low. Here, only 22% of the 13C glucose remained after 3 month, whereas 40% remained in the fertilized tree treatment. Only small proportions of the recovered 13C were present in the pool of water extractable C (<1%) and in living microbial biomass (8±3%, 3 days after glucose addition). As protection by clay minerals and aggregates is likely not a relevant process in this sandy soil, we suspect the remaining 13C to be stabilized in microbial residues, but depending on N demand. We assume that microbial necromass accounts for a considerable proportion to total SOC storage, especially under conditions of adequate nitrogen supply.

How to cite: Meyer, N., Sietiö, O.-M., Adamczyk, S., Biasi, C., Ambus, P., Mganga, K., Shrestha, R., Kalu, S., Martin, A., Glaser, B., and Karhu, K.: Stabilization of labile carbon in soil microbial biomass and necromass – A question of nitrogen deficiency?, EGU General Assembly 2020, Online, 4–8 May 2020, EGU2020-7121, https://doi.org/10.5194/egusphere-egu2020-7121, 2020