Chemotaxis under flow disorder shapes microbial dispersion in porous media

Natural soils are host to a high density and diversity of microorganisms, and even deep-earth porous rocks provide a habitat for active microbial communities. In these environ- ments, microbial transport by disordered flows is relevant for a broad range of natural and engineered processes, from biochemical cycling to remineralization and bioremediation. Yet, how bacteria are transported and distributed in the sub- surface as a result of the disordered flow and the associ- ated chemical gradients characteristic of porous media has remained poorly understood, in part because studies have so far focused on steady, macroscale chemical gradients. Here, we use a microfluidic model system that captures flow disorder and chemical gradients at the pore scale to quantify the transport and dispersion of the soil-dwelling bacterium Bacillus subtilis in porous media. We observe that chemotaxis strongly modulates the persistence of bacteria in low-flow regions of the pore space, resulting in a 100% increase in their dispersion coefficient. This effect stems directly from the strong pore-scale gradients created by flow disorder and demonstrates that the microscale interplay between bacterial behaviour and pore-scale disorder can impact the macroscale dynamics of biota in the subsurface.