Quantifying whole tree non-structural carbon dynamics under long-term experimental drought using radiocarbon

Under increasingly frequent, persistent, and severe drought events, predicting future forest carbon dynamics necessitates quantitative understanding of the physiological processes leading to tree mortality and physiological impairment. The responses of non-structural carbon (NSC; primarily sugars and starch) pools in mature trees is particularly important, as dynamics in NSC interact with hydraulic damage to perturb future tree growth. However, NSC concentration measurements alone are not suUcient to understand the stress responses of tree NSC pools formed over years to decades. Thus, we are using radiocarbon (14C) to quantify the age of NSC stored within, and used by, piñon pine trees exposed to either severe or long-term drought stress at the Sevilleta LTER, in New Mexico, USA. Measuring the age of NSC allows inference on the storage history of a tree, and how different NSC pools may be altered by drought. Experimental plots are subjected to either 0% (control) or 90% reduction in precipitation. A 45% precipitation reduction plot has also been in place since 2009, offering a chance to study the impacts of a decade of drought. We are measuring Δ14C of NSC in twigs, bole sapwood, and coarse roots, as well as in CO2 respired from the bole and branches. Our goal is to quantify the role of different-aged NSC pools across tree organs in driving whole-tree physiological responses to drought. Preliminary results show that the long-term droughted trees store and respire on average younger NSC than control trees. Ongoing drought treatments and sampling will provide additional information on how NSC dynamics in these trees are influenced by drought.