Effect of conservation agriculture practices on the resilience of Mediterranean soils to the predicted seasonal drought events

How climate change will affect soil functioning is a major concern, especially in Mediterranean agrosystems, where, according to climate change projections, the occurrence of extreme temperatures and drought events will be increased. The main objective of our experiment was to evaluate the effect of land management (tillage system) on soil resilience against a simulated dry-rewetting cycle. Soil samples were collected from an in-situ field experiment established in 2008 in the Guadalquivir Valley, where conservation agriculture practices have been tested. Three different land management practices under a typical Mediterranean wheat-legume rotation system were compared: 1) traditional tillage (TT), 2) minimum tillage (MT) and 3) no-tillage (NT). Following our hypothesis, conservation agriculture practices (reduced tillage and no-tillage) may allow a more mature soil microbial community by reducing soil perturbation, and this would result in higher resistance of soil functioning against drought periods. Soil enzyme activities (β-glucosidase, phosphatase, acetylglucosaminidase, aminopeptidase, and dehydrogenase activities), microbial functional diversity (Microresp method), and soil DNA concentration (as an index of microbial biomass) were analyzed in a base-line sampling. Afterwards, a dry-rewetting cycle was simulated under controlled conditions. 8 subsamples of 50g from each soil sample were hydrated to reach 70% of each soil water holding capacity (WHC) and kept in those conditions for a pre-incubation period of 15 days. After this period, half of the replicates were let dry for 12 days (drought), while the others were maintained at 70% WFC (controls). Finally, all replicates were rehydrated again to the initial water content during a 14 days rewetting period. During this cycle, soil respiration rates were periodically measured to study the evolution of soil microbial activity. Our results showed that initial respiration rates were slightly higher in MT compared to NT (p<0.1), likely due to higher organic C and N content in the MT soils. Drought extremely reduced respiration rates in the three treatments, but the results did not show a clear pattern among treatments. During the rewetting period, respiration rates were significantly higher in drought samples in comparison with the controls, while no significant differences were found for the land management treatments. Besides, land management practices did not have a significant effect on soil DNA concentration, functional diversity of the microbial community, or enzyme activities. To conclude, the absence of a clear effect of land management practices on soil resilience to drought may be due to the experimental conditions. An in-situ experiment will allow us to determine if tillage reduction enhances soil resilience to moisture stress.