Soil structural development in a rehabilitated open-cast mine site in south-east Australia

Rehabilitated soils from post mining fields are considered to have poor soil structure, low nutrient content and microbial activity. Soil development during rehabilitation is a complex biogeochemical process influenced by the inherent properties of the substrate used for the rehabilitation. Besides disturbed soil properties, in Australia soil rehabilitation success is also influenced by climatic conditions like high evaporation rate which affects rebuilding of soil system functions. There are several studies looking into the development of soil properties post rehabilitation in temperate climates, however, the intertwined development of soil structure, quality and quantity of soil organic matter (SOM) after the rehabilitation under water stressed environment is not clear until now.

In this study, we used a space-for-time chronosequence approach in the rehabilitated open-cast mine site at Yallourn (Victoria, Australia) to elucidate the development of soil structure and soil organic matter after rehabilitation. We selected five different fields with increasing rehabilitation ages (2, 3, 10, 21 and 39 years) and two mature soils that are used as grazing land. In each field, we sampled 6 independent locations with stainless steel cylinders (100 cm³) at two depths of 0-4 cm and 10-14 cm.  All samples were analysed for bulk density, organic carbon (OC) and total nitrogen (TN) concentration. Selected samples were wet sieved into four aggregate size classes of <63 µm, 63-200 µm, 200-630 µm and >630 µm. Each aggregate size class was characterized by OC and TN concentration. The chemical composition of the SOM of selected samples was characterized using solid-state ¹³C NMR spectroscopy.

The studied soils have a strong temporal dynamic and variability as determined for the soil properties bulk density and SOM stocks. Aggregate fractionation showed that large macroaggregates (>630 µm) were the most abundant size class fractions in each rehabilitation field, representing 95-75% of the total soil mass. SOM played an important role in the formation of large macroaggregates, where the highest contribution to total OC content was observed. It became evident that plant derived carbon had a decisive role in the structural formation, because O/N-alkyl-C and alkyl-C chemical shift regions represented the highest relative intensities throughout the chronosequence.