EGU21-9524, updated on 04 Mar 2021
https://doi.org/10.5194/egusphere-egu21-9524
EGU General Assembly 2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes

Jiachao Zhou1, Xiaofen Chai1, Lin Zhang1, Timothy George2, Fei Wang3, and Gu Feng1
Jiachao Zhou et al.
  • 1College of rResource and Environmental Science, China Agricultural University, Beijing, China (zhjchao1990@126.com)
  • 2The James Hutton Institute, Invergowrie, Dundee, United Kingdom
  • 3School of Resource and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang, China

Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species, which vary in morphological, physiological, and genetic traits. This colonization constitutes the mycorrhizal nutrient uptake pathway (MP) and supplements the pathway through roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of the microbiome on the extraradical hyphae differ between AM fungal species remains unknown. In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a splitroot compartmented rhizobox cultivation system, which allowed us to inoculate two AM fungal species separately in two root compartments, was used. We inoculated two separate AM fungal species combinations, (i) Funneliformis mosseae and Gigaspora margarita and (ii) Rhizophagus intraradices and G. margarita, on a single root system of cotton. The hyphal exudate-fed, active microbiome was measured by combining 13C-DNA stable isotope probing with MiSeq sequencing. We found that different AM fungal species, which were simultaneously colonizing a single root system, hosted active microbiomes that were distinct from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. We conclude that the arbuscular mycorrhizal fungal component of the system is responsible for the recruitment of distinct microbiomes in the hyphosphere. We found that arbuscular mycorrhizal fungi cocolonizing on single plant roots recruit their own specific microbiomes, which should be considered in evaluating plant microbiome form and function. Our findings demonstrate the importance of understanding trophic interactions in order to gain insight into the plant-AM fungus-bacterium symbiosis

How to cite: Zhou, J., Chai, X., Zhang, L., George, T., Wang, F., and Feng, G.: Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes, EGU General Assembly 2021, online, 19–30 Apr 2021, EGU21-9524, https://doi.org/10.5194/egusphere-egu21-9524, 2021.

Corresponding presentation materials formerly uploaded have been withdrawn.