Role of iron-carbon interactions in the release of greenhouse gases from permafrost systems

As permafrost thaws, vast stocks of organic carbon previously accumulated within these systems are vulnerable to microbial decomposition and may be released as the greenhouse gases CO₂ and CH₄. The release of carbon from permafrost systems is expected to lead to runaway positive feedbacks. The timescale and magnitude of the permafrost-climate feedback is highly uncertain as knowledge gaps remain regarding the rate of decomposition of permafrost organic carbon. These knowledge gaps stem, in part, from poor understanding of the association between organic carbon (in the form of organic matter) and minerals, especially high surface area iron minerals. In this work, we investigated the coupling of iron and carbon cycles in permafrost peatlands and its effect on greenhouse gas release. We first showed that up to 20% of the organic carbon in intact permafrost sites may be associated with iron(III) (oxyhydr)oxides and thereby protected from microbial decomposition. At the onset of thaw, this association is broken down, likely due to the microbial reduction of iron(III), and previously protected carbon is thus released. Using microbiological and molecular biological tools, we linked this breakdown to an increase in the abundance of methanogenic microorganisms and concentrations of methane. Preliminary work also suggests that part of the released organic carbon may re-associate with dissolved iron in thaw ponds to form flocs. Currently, we are investigating the molecular composition of organic matter as it undergoes these redox processes with the goal of linking bioavailability to composition. We complement this work with enrichment experiments and microbial community analyses to determine the microbial key players controlling iron(III) reduction and the potential for subsequent microbial Fe(II) oxidation. Collectively, the results of this project suggest that upon thawing, organic matter previously associated with minerals is mobilized and is likely susceptible to microbially-mediated release as CO₂ and CH₄.