Cytotoxicity as a proxy for particle-associated and dissolved organic contaminant loads in rivers during floods

Storm events lead to a mobilization of dissolved and particle-associated organic pollutants that pose a risk to river ecosystems. Target screening can hardly capture the broad range of compounds present in stormwater and receiving streams. Thus, an additional monitoring proxy that describes the overall chemical load in stormwater is needed. Each chemical in a mixture contributes, albeit with different potency, to cytotoxicity measured by reduction of cell viability after 24h in four human cell lines. Thus, the aim of this study was to investigate the applicability of cytotoxicity as a proxy for the organic contaminant load of rivers during storm events. Field investigations took place in the Ammer River (annual average discharge 0.87 m³ s^-1) close to Tübingen, Germany, during intense precipitation events in June 2021. The sampling site was located at the outlet of the gauged catchment (134 km²), thus, integrating inflowing water from all upstream tributaries and sewer overflows. During storm events, high-resolution temporal monitoring of discharge, suspended particles, particle characteristics, as well as dissolved and particle-associated organic contaminants was conducted using both chemical analyses and cell-based in vitro bioassays. The cytotoxicity in the water phase (expressed as toxic units, TU), was similar among the cell lines. The TU flux followed the course of the hydrograph with highest values at the maximum or slightly after the discharge peak. This finding suggests that the chemical load is controlled by the transported volume of water despite the fact that different contaminant sources are likely to contribute to the water flux and pollutant load in the river at different time points of the hydrograph. For the particle-associated cytotoxicity, the TU flux also followed the course of the events suggesting that the particle-associated cytotoxicity in the river is, similar to the water cytotoxicity, controlled by the particle load in the river. This highlights that the cytotoxicity is a suitable proxy to detect mixtures of organic compounds and, thus, assess the chemical load in rivers during storm events.