EGU General Assembly 2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.

Mapping the organic N distribution in the rhizosphere of maize

guoting shen1, Sajedeh Khosrozadeh1, Negar Ghaderi1, Andrey Guber2, and Evgenia Blagodatskaya1
guoting shen et al.
  • 1Department of Soil Ecology, Helmholtz Centre for Environmental Research – UFZ, Halle (Saale), Germany
  • 2Department of Soil, Plant and Microbial Sciences, Michigan State University, 48824, East Lansing, MI, USA

The combination of two non-destructive 2D imaging methods: amino-mapping and zymography have been developed and applied to monitor organic nitrogen allocation in the rhizosphere of Zea mays L.  under contrasting nutrient treatments. Amino-mapping was based on the fluorescent reaction of o-phthaldialdehyde and β-mercaptoethanol enabling to estimate the content of labile organic N, playing an important role in soil nitrogen cycling. Amino-mapping was coupled with leucine-aminopeptidase zymography to quantify the amino-N release in the rhizosphere of maize grown under climate chamber conditions for 3 weeks. The combination of the two approaches enabled visualization of organic N hotspots either distinctly separated or overlapped with the hotspots of enzymatic activity. This work was conducted within the framework of the priority program 2089 “Rhizosphere spatiotemporal organization – a key to rhizosphere functions”, funded by German Research Foundation (DFG – Project number: 403664478). Seeds of the maize were provided by Caroline Marcon and Frank Hochholdinger (University of Bonn).

How to cite: shen, G., Khosrozadeh, S., Ghaderi, N., Guber, A., and Blagodatskaya, E.: Mapping the organic N distribution in the rhizosphere of maize, EGU General Assembly 2022, Vienna, Austria, 23–27 May 2022, EGU22-9125,, 2022.