Hydrogels that Co-Encapsulate Slow Release Compounds and Rhodococcus rhodochrous ATCC 21198 for the Aerobic Cometabolic Treatment of 1,4-Dioxane and CAHs

A novel aerobic process has been developed for the passive cometabolic treatment of contaminant mixtures in groundwater.  Our studies have focused on Rhodococcus rhodochrous ATCC 21198 that can concurrently oxidize 1,4-dioxane (1,4-D) and diverse mixtures of chlorinated aliphatic hydrocarbons (CAHs). We found that the short chain alkane monoxygenase (SCAM) responsible for the cometabolism of the contaminants is induced after grown on 1-butanol and 2-butanol, permitting the use of Slow Release Compounds (SRCs) that slowly hydrolyze to produce these alcohols.  Methods were developed to co-encapsulate the SRCs and ATCC 21198 in gellan-gum hydrogel beads. ATCC 21198 grows within the gellan-gum beads and upon diffusion into the beads the contaminants are transformed. In batch reactors containing the gellan-gum beads successive additions of a mixture of  1,1,1-trichloroethane (1,1,1-TCA_, cis-dichloroethene (cis-DCE), and 1,4-D were transformed for over 300 days, with the rates of cometabolism correlated with the rates of alcohol release, oxygen consumption and CO₂ production. Continuous flow tests have been performed with columns packed with the gellan-gum beads. The columns mimic passive treatment that might be achieved using an in-situ permeable reactive barrier (PBR) constructed with the gellan-gum beads.  Over 99% removal of a mixture of 1,1,1-TCA, cis-DCE, and 1,4-D, each at influent concentration of 250 µg/L, was achieved with a hydraulic residence time of approximately 12 hours  The columns effectively transformed the contaminant mixture for over 600 pore volumes (300 days). The columns performance was negatively affected when cis-DCE was replaced by 1,1-dichloroethene (1,1-DCE), due to 1,1-DCE transformation product toxicity.  The column containing the SRC that produced 2-butanol was more negatively impacted by 1,1-DCE due to the lower biomass that developed in the gellan-gum beads. Studies are currently being performed in a 3-D physical aquifer model using a funnel-and-gate system with the co-encapsulated gellan-gum beads used to create a cometabolic permeable reactive barrier.