Nitrogen modeling and performance of Multi-Soil-Layering (MSL)bioreactor treating domestic wastewater in rural community

The multi-soil-layering (MSL) bioreactor has been considered in the latest research as an
innovative bioreactor for reducing the level of pollutants in wastewater. The efficiency of the
MSL bioreactor towards nitrogen pollution is due to the mineralization of organic nitrogen in
aerobic layers to ammonia, and reactivity of ammonia nitrogen with soil and gravel by its
adsorption into soil layers followed by nitrification and denitrification processes when the
alternating phases of oxygenated/anoxic conditions occurs in the filter. In this study, we have
examined the performance of the MSL bioreactor at different hydraulic loading rates (HLRs)
and predicted the removal rate of nitrogen. To improve the prediction accuracy of the models,
the feature selection technique was performed before conducting the Neural Network model.
The results showed a significant removal (p <0.05) efficiency for five-day biochemical
oxygen demand (BOD 5,  86%), ammonium (NH 4 + , 83%), nitrates (NO 3 − , 81%), total kjeldahl
nitrogen (TKN, 84%), total nitrogen (TN, 84%), orthophosphates (PO 4 3− , 91%), and total
coliforms (TC, 1.62 Log units). However, no significant change was observed in the nitrite
(NO 2 − ) concentration as it is an intermediate nitrogen form. The MSL treatment efficiency
demonstrated a good capacity even when HLR increased from 250 to 4000 L/m 2 /day,
respectively (e.g., between 64% and 86% for BOD 5 ). The HLR was selected as the most
significant (p < 0.05) input variable that contribute to predict the removal rates of nitrogen.
The developed models predict accurately the output variables (R 2  > 0.93) and could help to
investigate the MSL behavior.