Intensified warming effects on soil respiration upon thermokarst formation

As global temperatures continue to rise, a key uncertainty of terrestrial carbon (C) climate feedback is the rates of C loss upon abrupt permafrost thaw. This type of thawing - termed thermokarst - may in turn accelerate or dampen the response of microbial degradation of soil organic matter and carbon dioxide (CO₂) release to climate warming. However, such impacts have not yet been explored in experimental studies. Here, by experimentally warming three thermo-erosion gullies in an upland thermokarst site combined with incubating soils from another five thermokarst-impacted sites on the Tibetan Plateau, we investigate whether and how abrupt permafrost thaw would influence the responses of soil CO₂ release to climate warming. Our results show that warming-induced increase in soil CO₂ release is higher in thermokarst features than the adjacent non-thermokarst landforms. This larger warming response is mainly attributed to the lower substrate quality and higher abundance of microbial functional genes for recalcitrant C degradation in thermokarst-affected soils. Taken together, our study provides experimental evidence that abrupt permafrost thaw aggravates the warming-associated soil CO₂ loss, which will exacerbate the positive soil C-climate feedback in permafrost-affected regions under future warming scenarios.