Selective pressure of atrazine on bacterial pattern in a hypersaline lake environment

The attenuation of atrazine in the saline water of the hypersaline Pétrola lake from a natural reserve (SE Spain) was studied to get more insight into the processes governing the fate of the contaminant in highly saline environments. In microcosms, the water column was spiked with 15.4 mg/L of atrazine for 24 days. Before atrazine amendment, the initial distribution of bacterial community was mostly composed of Proteobacteria (25.5 %), Cyanobacteria (25.2 %), Actinobacteriota (18.5 %), Verrucomicrobiota (11.5 %) and Bacteroidota (10.1 %). Within the mayor phyla, the most abundant families were identified as Cyanobiaceae (25 %), Rhodobacteraceae (10 %), Alcaligenaceae (9.9 %), Microbacteriaceae (7.3 %) and a poorly described PeM15 (6.2 %).

The reduction of atrazine concentration in the water column reaches 86.9 %, which means a reduction of dissolved atrazine mass of 98.1 %. Parallel to the decrease in atrazine, the amount of its intermediate degradation metabolite, desethylatrazine, increased. Desethylatrazine was the major short-term metabolite within the first 8-12 days, indicating the potential activity by atrazine-degrading bacteria. No deisopropylatrazine was detected in the saline water above detection limit. Microbiology results showed that atrazine can be removed from the saline lake environment. After atrazine amendment, the taxonomic bacterial composition at phylum level shifted to Proteobacteria (60.8 %), Patescibacteria (9.7 %), Bacteroidota (7.3 %), Campylobacterota (6.4 %) and Actinobacteriota (2.1 %). Atrazine supplementation suggested a selective pressure on bacterial structure morphology through the emergence of different dominant groups (i.e., Campylobacterota) or even the eradication of those phyla of bacteria capable of photosynthesis (i.e., Cyanobacteria). At family level, Rhodobacteraceae (16.7 %), Burkholderiaceae (11.7 %), Thiomicrospiraceae (7.9 %), Methylophagaceae (6.7 %), Sulfurimonadaceae (4.4 %) and Solimonadaceae (3.3 %) were the most abundant in the water column at the end of the experiment.

Related atrazine-degrading families established at the end of the experiment were Rhodobacteraceae, Solimonadaceae, Pseudomonadaceae, Rhizobiaceae, Chromatiaceae, Bacillaceae, Xanthomonadaceae, Caulobacteraceae, Moraxellaceae, Streptomycetaceae, Microbacteriaceae and Nannocystaceae. Related candidates such as Pseudomonas paralactis, Microbacterium sp., or Arthrobacter sp., among others, were isolated in water samples from previous studies. The bacterial candidates for atrazine degradation identified in the water column indicate that the herbicide acted as a selective pressure factor, altering the composition of the bacterial pattern. The water column would constitute a reactive environment which may govern the fate of pesticides in saline surface water bodies.