Separation of plastic degrading bacteria from biofilm using electromagnetic radiation by switch off quorum sensing

Quorum sensing has used to achieve high cellular density by which a bacterial community makes biofilm on a substrate. Intensity of bioluminescence per microbial cell is constant at high culture density however it decreases considerably at low culture density. Based on this fact, it is hypothesized that bioluminescence switch off quorum sensing regulation among the microbial community once high cellular density was achieved; as bioluminescence requires energy and microbial systems are highly regulated, microbes does not spend energy for process which is of no use. Multispecies microbes involved in biofilm formation on plastic surface can be unsocialized by exposing to different energies (wavelength) of electromagnetic radiations in a novel experimental setup. Detailed output parameters that statistically performed include: (a) DNA quantity (85% to 98% more DNA was obtained in light treated samples compared to control), (b) total bacterial count using DAPI dye by epifluorescence (2.8×10⁵, 6.1×10⁵, 6.7×10⁵ for control, blue light treated, red light treated samples respectively after 48 hours of treatment) and (c) cultivation of plastic-degrading microbes on modified Bushnell Hass agar (obtained microbial growth for blue and red light treated samples but no growth for control samples). Metagenomics DNA sequencing analysis revealed that various microbial species respond to electromagnetic radiation of specific wavelength and compare it with control that is microbes recover in without light treatment setup that is loosely attached microorganisms from plastic biofilm which may not be directly involve in plastic degradation. These loosely attached microorganisms must show symbiotic relation with actual plastic degrading microorganisms (recover by electromagnetic radiation treatment). Based on experimental findings, microbial quorum sensing can successfully regulated and detach multispecies plastic-degrading biofilm to be used for further analysis or treatment by electromagnetic radiations.