Stratigraphic changes in size and shape of calcareous microplankton near the Tithonian–Berriasian boundary are a key indicator of paleoenvironmental changes in Tethyan realm. Changes in calpionellid size occurred in parallel with a significant increase in the calcareous nannoplankton abundance. This process is associated with the shift of the carbonate production maximum from shallow shelves to the open ocean. These calpionellid “blooms” are not an isolated phenomenon, but part of a broader restructuring of ocean ecosystems. Although the so-called “Calpionella alpina bloom event” (characterized by high abundance of the small spherical forms) has been known for a long time, its exact dynamics and relationship to biostratigraphic boundaries have not yet been quantitatively processed at high stratigraphic resolution.
The aim of this study was to verify, through high-resolution calpionellid morphometry, whether this bioevent is a one-time event or a recurrent process, and to determine its exact position relative to biostratigraphic markers, especially relative to the latest occurrences of the genus Crassicollaria.
The samples were obtained from the pelagic carbonate succession at Brodno (Pieniny Klippen Belt, Western Carpathians). We measured the loricae length (Ll) and width (Wl) in thin-sections using light microscopy. Out of a total of 15,081 individuals; 7,833 were analysed after filtering (exclusion of oblique sections). Advanced statistical methods were used to identify trends: “punctuation” models characterized by intervals of stasis that are separated by sudden jumps, SiZer analysis (for stability of trends in size or shape at different stratigraphic resolutions) and principal coordinate analyses (PCO) assessing variability in increment-specific size and shape distributions.
Our analysis revealed three separate minima in lorica size. The first minimum (the base of the Remanei Subzone) was not accompanied by changes in shape (Ll ~50 µm). The second minimum is located near the Intermedia/Colomi Subzone (Ll ~60 µm). This minimum corresponds to the first Calpionella alpina bloom interval, which precedes the lower boundary of the Calpionella Zone as detected by Michalík et al. (2009, 2021). The third minimum occurs in the lowermost part of Alpina Subzone (the second Calpionella alpina bloom interval), which is characterized by small and spherical calpionellid forms. These three minima of size reduction are clearly separated by periods of larger individuals (Ll ~80 µm). The synchronous response of the genera Calpionella and Crassicollaria to these changes suggests that the entire community was responding to the same oceanographic triggers, probably changes in temperature or nutrient availability.
Our study demonstrated that a quantitative approach allows for a finer biostratigraphic subdivision and a better interpretation of the evolutionary dynamics of microplankton. The synchronized response of different groups of microplankton to environmental disturbances (e.g. warming) is often manifested by a body size decrease. In future, we propose to combine these morphometric data with calcareous nannofossils, calcareous dinoflagellate cysts high-resolution analysis and geochemical proxy data (Mg/Ca), which could clarify whether the observed calpionellid size minima are linked to specific phases of nannoplankton expansion and changes in the global carbon cycle.
This study was supported by the Slovak Research and Development Agency (APVV 22/0523).