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biofilms 9 - online conference | 29 September - 1 October 2020
biofilms9-151
https://doi.org/10.5194/biofilms9-151
biofilms 9 conference
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Antimicriobial Activity of Silver-Nanoparticles studied by Scanning Probe Microscopy

Giada Caniglia1, Anna Heinzmann1, Maria Chiara Sportelli2, Antonio Valentini3, Nicola Cioffi2, and Christine Kranz1
Giada Caniglia et al.
  • 1Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee, 11 – 49081 Ulm, Germany
  • 2Chemistry Department, University of Bari “Aldo Moro”, V. Orabona, 4, 70126, Bari, Italy
  • 3Physics Department, University of Bari “Aldo Moro”, V. Orabona 4, 70126, Bari, Italy

Biofilms are well-organized sessile communities which exhibit an increased tolerance against antimicrobial and antibiotic treatments in comparison with their planktonic counterparts. Biofilms are ubiquitous and due to their high resilience, the problem with contamination of medical implants leads to serious health problems [1]. Within the last decades, novel therapies to prevent the formation of biofilms have been developed and, among others, antimicrobials based on metal nanoparticles (NPs) have been intensively studied [2], due to their ability to reduce biofilm formation. Silver nanoparticles (AgNPs) are known to be effective antimicrobial agents, as silver(I) has the ability to penetrate the cell and produce oxidative stress via the generation of reactive oxygen species (ROS) [3]. To understand the release mechanism of silver(I) ions, scanning electrochemical probe microscopy such as scanning electrochemical microscopy (SECM) is highly suitable.

In this contribution, biocompatible AgNPs-fluoropolymer (Ag-CFx) composite films, prepared by ion beam sputtering (IBS) deposition [4], are investigated in respect to silver(I) release associated to the swelling of the antimicrobial film. The mechanism of the silver(I) release is studied real-time by scanning electrochemical microscopy (SECM) in combination with square-wave stripping voltammetry and the relation between controlled silver(I) release and the swelling of Ag-CFx films will be presented, combining electrochemical techniques and atomic force microscopy (AFM).

 

References

[1] G.C. Anderson, et al. Innate and Induced Resistance Mechanisms of Bacterial Biofilms. In Bacterial Biofilms; Romeo, T., Ed.; Springer Berlin Heidelberg: Berlin, Heidelberg, 2008; pp 85–105.

[2] M.C. Sportelli, et al. Nano-Antimicrobials Based on Metals. In Novel Antimicrobial Agents and Strategies; John Wiley & Sons, Ltd, 2014; pp 181–218.

[3] N. Durán, et al. Nanomedicine, 2016, 12(3), 789-799.

[4] M.C. Sportelli, et al. Sci. Rep. 2017, 7 (1), 11870.

 

Acknowledgements

Financial support is acknowledged from European Union’s 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 813439.

How to cite: Caniglia, G., Heinzmann, A., Sportelli, M. C., Valentini, A., Cioffi, N., and Kranz, C.: Antimicriobial Activity of Silver-Nanoparticles studied by Scanning Probe Microscopy, biofilms 9 conference, Karlsruhe, Germany, 29 September–1 Oct 2020, biofilms9-151, https://doi.org/10.5194/biofilms9-151, 2020