EGU2020-11264
https://doi.org/10.5194/egusphere-egu2020-11264
EGU General Assembly 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Tracing ecosystem scale interactions of volatile organic compound (VOC) and CO2 emissions by position-specific and whole ecosystem isotope labelling

Christiane Werner1, Nemiah S. Ladd1, Laura Meredith2, and the B2 WALD*
Christiane Werner et al.
  • 1Freiburg, Faculty of Enviroment and natural resources, Ecosystem Physiology, Freiburg, Germany (c.werner@cep.uni-freiburg.de)
  • 2University of Arizona
  • *A full list of authors appears at the end of the abstract

Ecosystem processes present a complex interplay between different components, such as vegetation, soil, and the rhizosphere. All these different components can emit (or even uptake) a plethora of volatile organic compound (BVOC) with highly dynamic response to environmental changes. However, processes controlling carbon allocation into primary and secondary metabolism such as VOC synthesis or respiratory CO2 emission remain unclear. De novo synthesis of BVOC depends on the availability of carbon, as well as energy provided by primary metabolism. Thus, carbon allocation may compete between primary and secondary metabolism, which are linked via a number of interfaces including the central metabolite pyruvate. It is the main substrate fulling respiration, but also a substrate for a large array of secondary pathways leading to the biosynthesis of many volatile organic compounds, such as volatile isoprenoids, oxygenated VOCs. Within the European Research Council (ERC) Project VOCO we developed a novel technological basis to couple CO2 fluxes with VOC emissions based on simultaneous real-time measurements of stable carbon isotope composition of branch, root, and soil respired CO2 and VOC fluxes (Fasbender et al. 2018). Position specific 13C-labeled pyruvate feeding experiments are used to trace partitioning within the metabolic branching points into VOCs versus CO2 emissions, bridging scales from sub-molecular to whole-plant and ecosystem processes. Positional 13C-labelling will trace real-time sub-molecular carbon investment into VOCs and CO2, enabling mechanistic descriptions of the underlying biochemical pathways coupling anabolic and catabolic processes.

To trace ecosystem scale interactions, we implemented a whole-ecosystem labelling approach in the world’s largest controlled growth facility: the Biosphere 2 Tropical Rainforest. In the Biosphere 2 Water, Atmosphere, and Life Dynamics (B2-WALD) experiment, we applied an ecosystem scale drought and tracing carbon allocation and dynamics of VOC, CO2 and H2O fluxes from leaf, root, soil and atmospheric scales. The overarching goal of B2-WALD is to track, biological mechanisms controlling the fate of CO2, VOC and water cycling in an ecosystem under change in an interdisciplinary approach. This comprehensive data set will be used for carbon and water partitioning from the metabolic to ecosystem scale

Fasbender L., et al. (2018). A novel approach combining PTR-TOF-MS, 13CO2 laser spectroscopy and 13C-metabolite labelling to trace real-time carbon allocation into BVOCs and respiratory CO2. PLOS One,13: e0204398

 

B2 WALD:

John Adams, Roya Aminitabrizi, Michael Bahn, Xuejuan Bai, Kinzie Bailey, Ian Baker, Ines Bamberger, Matthias Beyer, Daniel Blomdahl, Kevin Bonine, Aaron Bugaj, Michael Burman, Melissa Burnett, Vanessa Buzzard, Joseph Byron, Teverrick Chee, Megan Claflin, Marissa Clover, Róisín Commane, Rachel Cruz-Perez, Charlie Cunningham, Erik Daber, Adrian Dahlman, Justin Davis, Jason Deleeuw, Michaela Dippold, Katerina Dontsova, Maren Dubbert, Emiley Espelage, Jane Fudyma, Andrea Ghirado, Juliana Gil-Loaiza, Jess Graham, Emma Hall, Henrik Hartmann, Nina Herz, Rolf Hofmann, Linnea Honeker, Jia Hu, Jianbei Huang, Halley Hughes, Ed Hunt, Bonnie Hurwitz,Johannes Ingrisch, Sydney Kerman, Thomas Klüpfel, Jordan Krechmer, Jürgen Kreuzwieser, Angelika Kübert, Kathrin Kühnhammer, Nemiah Ladd, Marco Lehmann, Brian Lerner, Ashia Lujano, Brenna McIntyre, Laura Meredith, Luke Miller, Pawel Misztal, Kathiravan Mohamed Meeran, Peter Moma, Patrick Murphey, Thor Neill, Wei-Ren Ng, Eva Pfannerstill, Alise Ponsero, Neill Prohaska, Gemma Purser, Joseph R. Roscioli, Joaquin Ruiz, Scott Saleska, Jörg-Peter Schnitzler, Anupama Sethuraman, Lingling Shi, Joanne Shorter, William K. Smith, Phoenix Spivey, Scott Stark, Malak Tfaily, Jana U'Ren, Joost van Haren, Xian Wang, Christiane Werner, Jonathan Williams, Connor Youngerman,

How to cite: Werner, C., Ladd, N. S., and Meredith, L. and the B2 WALD: Tracing ecosystem scale interactions of volatile organic compound (VOC) and CO2 emissions by position-specific and whole ecosystem isotope labelling, EGU General Assembly 2020, Online, 4–8 May 2020, EGU2020-11264, https://doi.org/10.5194/egusphere-egu2020-11264, 2020

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