Tracing dynamic water uptake and transport from root to canopy by online monitoring of water isotopes in an enclosed tropical forest in response to drought

Online (or: in situ) methods for measuring soil and plant water isotopes have been identified as an innovative and crucial step to address recently identified issues in studying water uptake using stable isotope techniques.

During a controlled three month drought and rewetting experiment at the Biosphere 2 (B2) enclosed rainforest, a recently developed online method for measuring stem water isotopes (Marshall et al., 2019), namely ‘stem borehole equilibration’, was combined with online monitoring of soil water isotopes and transpired water isotopes as well as sap flow and stem water storage. This enabled us to study root water uptake depths of different tree species and dynamic changes during the dry down and rewetting. After two months of drought, the system was supplied with isotopically labelled water (deuterated water) from down below via a pipe system spanning across the complete B2 rainforest in order to identify deep water uptake of the rainforest trees and hydraulic redistribution.

Results show that – as expected – all monitored trees responded to the drought by changing their root water uptake towards deeper soil depths while sap flow rates of most trees decreased. When rewetting the system, deep water uptake from the base of B2 (between 2.5m and 4m soil depth) was identified in all large, mature trees (Clitoria faichildiana, Hibiscus tilliaceus, Hura crepitans, Pachira aquatica). No deep water uptake was found in the smaller trees (mainly Pachira aquatica). Furthermore, stem water storage was notably different between species and affected their adaptation to drought and response to rewetting. The labelled water was also identified in the transpired water more than one month after re-starting rainfall at B2.  However, no hydraulic redistribution was identified.

The holistic approach for monitoring the interactions of soils and plants provides inevitable insights into the adaptation of (enclosed) rainforests under drought and might have implications for natural rainforests. In particular, the capability of large trees to develop deep roots and the role of stem water storage are important elements for adaptation to climatic changes and need to be studied further under ‘real’ conditions.

References

Marshall, J.D., Cuntz, M., Beyer, M., Dubbert, M., Kühnhammer, K., 2019. Borehole equilibration: testing a new method to monitor the isotopic composition of tree xylem water in situ. Front. Plant Sci.