Effect of urease inhibitor and biofertilizer on wheat yield and related crop parameters

Agricultural production must increase by 50% to support about 9 billion people by 2050. Previous studies show that integrated crop-soil management strategies can improve cereal yield by 30% without increasing nitrogen use. Sustainable practices and the application of environmentally friendly technologies can help to reach this point by improving resource use efficiency and increasing yield. For this purpose, the effect of urease inhibitor and biofertilizer were evaluated in this study as environmentally friendly technologies that can increase cereal grain yield.

In the spring of 2022, a field experiment was established at the experimental station of the University of Natural Resources and Life Sciences (BOKU), located in the east of Vienna, to determine the effect of urease inhibitor and biofertilizer on wheat production. A randomized complete block design including five treatments and four replicates was used in this study. Each main plot was 9 by 9 meters, and a buffer zone of 1.5 meters was implemented between each of the individual main plots. The treatments were: T₁ (control treatment - without N fertilizer), T₂ (Urea only), T₃ (Urea+Urease Inhibitor (UI)), T₄ (Urea+Biofertilizer), T₅ (Urea+UI+Biofertilizer). All treatments received 50 kg N ha^-1 at tillering stage (GS 31), except T₁. In this study N-(n-butyl) thiophosphoric triamide (nBTPT) or “Agrotain” was used as UI and Azotobacter chroococcum or “AZOTOHELP” was applied as biofertilizer. To determine wheat yield (grain and straw), a 1.5 by 8 meter area was harvested in each main plot (9 by 9 meters). To measure other parameters including the number of tillers per square meter, 1000-grain weight (g), plant height (cm), spike length (cm) and numbers of grains per spike, a 1m-by-1m area was harvested within each main plot for all treatments.

The highest grain and straw yields were observed in the Urea+UI+Biofertilizer treatment, with a grain yield of about 20, 11, 8% higher, compared to the Urea, Urea+UI and Urea+Biofertilizer treatments, respectively. However, a significant difference in grain and straw yields was only observed between Urea and Urea+UI+Biofertilizer treatments. The grain and straw yields in the Urea+UI and Urea+Biofertilizer treatments were not significantly different from both Urea and Urea+UI+Biofertilizer treatments. The number of grains per spike and the weight of 1000-grain in the Urea+UI+Biofertilizer treatment showed an increase of about 20 and 11% respectively, compared to the Urea treatment, but these increases were not significant. Plant height in treatments that received nitrogen fertilizer was not affected by fertilization treatments, but spike length was affected. This study suggests that the use of urea fertilizer coated with urease inhibitor in combination with biofertilizer is a promising way for sustainable crop production in the lowlands of Austria.