Getting Out from Under: The Belowground Response of a Restored Grassland to Soil Disturbance and Resource Addition

Disturbances resulting from anthropogenic global change pose ongoing threats to plant biodiversity. Functional trait-based approaches enable ecologists to observe species-level stress responses with implications for community-level adaptations to disturbances. DRAGNet (Disturbance and Resources Across Global Grasslands) leverages grassland restoration to explore the mechanisms driving disturbance recovery and community assembly. In this single-site study, we examine how plant composition and traits vary across disturbance (tillage) and soil resource (NPK+) gradients. Plant composition will be surveyed in 28 plots, with root and soil samples extracted for trait analysis and soil nutrient testing. We predict that plants in disturbed, nutrient-enriched plots will exhibit divergent functional traits, including reduced root biomass and specific root length, alongside changes in above-ground traits. Preliminary data illustrates the impact disturbance can have on community composition, particularly by promoting invasive species (PERMANOVA, p = 0.0576). This finding underscores the influence of disturbance on plant community assembly and highlights the potential vulnerability of restored grasslands to invasive species proliferation under human-induced disturbances. This study aims to uncover the root functional traits driving the recovery of a restored grassland across both soil disturbance and resource gradients.