Rogoznica Lake–Dragon Eye (RL) is a unique, highly eutrophic, stratified euxinic marine system on the Adriatic coast, sensitive to dynamic environmental shifts. Key physico-chemical transformations in the lake, directly driven by environmental changes, include water column warming, deoxygenation, accumulation of reduced compounds such as toxic sulfides and ammonia, and a increased frequency of anoxic holomictic events, contributing to the pronounced eutrophication [1-2]. All these changes highly impacts organic matter (OM) dynamics and properties. Long-term studies of OM dynamics reveal the accumulation of particulate (POC) and dissolved (DOC) organic carbon, especially in the anoxic hypolimnion, with DOC concentrations ranging from 0.809 to 7.16 mgL-1 and POC from 0.572 to 10.5 mgL-1 [2]. Qualitative changes in OM are evaluated using normalized surface activity (NSA = SAS/DOC) achieved by monitoring of DOC and its surface activity, i.e. surface active substances (SAS) [2-4]. Further characterization of organic matter (OM) is achieved through the analysis of stable isotopes of light elements (13C/12C, 15N/14N, 34S/32S) and the C:N ratio in the POC fraction providing insights into the structure of phytoplankton communities, the sources and origins of OM, and its role in biogeochemical cycles, utilizing the isotope-ratio mass spectrometry (IRMS) method.
Preliminary results (δ¹³C, δ¹⁵N, δ³⁴S) from seasonal RL water column samples revealed isotope values ranging from -20.89 to -32.30 ‰ for δ¹³C, -8.07 to 7.24 ‰ for δ¹⁵N, and -10.83 to 21.74 ‰ for δ³⁴S, with noticeable seasonal shifts along the water column related to variable physico-chemical parameters, including salinity fluctuations, oxygen saturation, and atmospheric deposition. The position of the chemocline, which separates the surface oxic and bottom anoxic water layers, is distinctly observable from the δ³⁴S values, also showing pronounced seasonality. These findings suggest that the OM in the RL water column is predominantly autochthonous, largely derived from phytoplankton activity, with occasional allochthonous inputs, as indicated by the C:N ratio (ranging from 1.09 to 6.51), contributing to eutrophication. During holomictic events, when the otherwise stratified water column becomes mixed and anoxic throughout, isotope ratios point to the presence of bacterially-produced OM. The isotopic data, in conjunction with other organic matter parameters (DOC, POC, SAS, NSA), reveal qualitative and quantitative changes in the composition of OM, offering deeper insight into the influence of environmental shifts on OM dynamics within RL.
This work was result of research activities within the MARRES (IP-2018-01-1717) and the ISO-ZOKO (IP-2024-05-2377) projects.
[1] I. Ciglenečki, Z. Ljubešić, I. Janeković, M. Batistić, in R.D. Gulati, E.S. Zadereev, A.G. Degermendzhi (eds) “Ecology of meromictic lakes”. Springer 2017, Cham, p 125−154.
[2] Simonović, N., Dominović, I., Marguš, M., Matek, A., Ljubešić, Z., Ciglenečki, I. Sci. Total Environ. 863 (2023) 161076.
[3] I. Ciglenečki, I. Vilibić, J. Dautović, V. Vojvodić, B. Ćosović, P. Zemunik, N. Dunić, H. Mihajlović, Sci. Total Environ. 730 (2020) 139104.
[4] Simonović; N., Marguš, M., Paliaga, P., Budiša, A., Ciglenečki, I. Mediterr. Mar. Sci. 25(1) (2024) 160-178.