Nitrogen (N) is a central component of all organisms, making it a key element in the production of organic matter in lakes. However, excessive bioavailable N originating from waste water or fertilizer input can exacerbate eutrophication, reduce oxygen levels, and promote the growth of toxic algal blooms. Understanding the sources, sinks, and internal cycling of N in lacustrine environments, both today and in the past, is crucial for providing paleolimnological information that can be related to past environmental changes. Traditionally, past changes in the lacustrine N cycle have been reconstructed using bulk sedimentary N isotope (δ15N) analyses. While this approach is relatively inexpensive and fast, it is widely known that bulk sediment δ15N values can be biased by diagenetic alteration and external organic and inorganic (e.g., clay-bound) N input. Here, we introduce a proxy that is relatively new to limnogeology, overcomes these issues, and has successfully been applied in paleoceanographic reconstructions for over two decades: diatom-bound nitrogen isotope ratios. The siliceous shells (called frustules) of diatoms contain small amounts of organic matter, whose nitrogen isotope composition is thought to be protected from diagenetic alteration and bacterial degradation. Previous studies have shown that the diatom-bound N isotopic composition correlates with the N isotopic composition of nitrate in the surface waters, which the diatoms assimilated during growth. Since the N isotopic composition of surface-water nitrate directly depends on N inputs/outputs (i.e., size of N fluxes and their respective δ15N), as well as internal N cycling processes, which all have a distinct impact on the N isotopic signature, diatom-bound δ15N can be used to infer past changes in the lacustrine N cycle.
Here, we present the first lacustrine application of the diatom-bound N isotope paleo-proxy, reconstructing nutrient cycling and paleoenvironmental conditions over the past centuries in Swiss lakes. In Lake Lugano (Switzerland), for example, we demonstrated that denitrification and N2 fixation were tightly coupled during the peak eutrophication period, and that changing redox conditions in the sediment influenced the extent of N-isotopic alteration of the bulk sediment (Studer et al., 2024). Furthermore, we will present our team's recent efforts to further ground-truth the diatom-bound N isotope proxy, including (i) data from natural lacustrine environments that provide insights into the acquisition of the diatom-bound N isotope signature during diatom growth, and (ii) data from degradation experiments under controlled laboratory conditions that address the resilience of the diatom-bound N isotope signature against early diagenetic alteration.
References
Studer, A.S., Wörmer, L., Vogel, H., Dubois, N., Hinrichs, K.-U., Bartosiewicz, M., Lepori, F., Lehmann, M.F. 2024. First lacustrine application of the diatom-bound nitrogen isotope paleo-proxy reveals coupling of denitrification and N2 fixation in a hyper-eutrophic lake. Limnology and Oceanography, 69, 1797-1809. https://doi.org/10.1002/lno.12627